Yaba Aylin, Kayisli Umit, Tanriover Gamze, Demir Necdet
Akdeniz University School of Medicine, Department of Histology and Embryology, Umlupinar Street, 07070 Campus, Antalya, Turkey.
Int J Dev Neurosci. 2011 Apr;29(2):131-6. doi: 10.1016/j.ijdevneu.2010.12.004. Epub 2010 Dec 14.
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena/VASP protein family and was for the first time identified in human platelets. VASP plays a role for cell adhesion and cell migration because of E/DFPPPPXD/E amino acid sequence in its structure which gives reaction with focal adhesion proteins. In this study we suggest that VASP expression may have important role for neural cell migration, differentiation, axonal growth and angiogenesis during prenatal cerebral cortical development. Our aim is to detect VASP expression by means of immunohistochemistry and Western blot analysis in developing human neocortex and adult brain cortex (n=12 samples from first, second, and third trimesters and n=3 adult normal cerebral cortex). Our results suggest that VASP showed different immunostaining patterns between cerebral cortical plates in prenatal and adult human brain samples. We observed that the expression patterns of the VASP protein are clearly identified in fibers, cytoplasm of neural cells and endothelial cells of vessels. We detected that VASP indicates progressive expression from the adult brain to second trimester neocortexes. Therefore, we suggest that VASP may play a crucial role in the regulation of human neonatal cerebral cortical development.
血管舒张刺激磷蛋白（VASP）是Ena/VASP蛋白家族的成员，首次在人血小板中被鉴定出来。由于其结构中的E/DFPPPPXD/E氨基酸序列能与粘着斑蛋白发生反应，VASP在细胞粘附和细胞迁移中发挥作用。在本研究中，我们认为VASP表达可能在产前大脑皮质发育过程中的神经细胞迁移、分化、轴突生长和血管生成中发挥重要作用。我们的目的是通过免疫组织化学和蛋白质印迹分析检测发育中的人类新皮质和成人脑皮质（来自第一、第二和第三孕期的12个样本以及3个成人正常脑皮质样本）中的VASP表达。我们的结果表明，VASP在产前和成人脑样本的大脑皮质板之间表现出不同的免疫染色模式。我们观察到，VASP蛋白的表达模式在纤维、神经细胞的细胞质和血管内皮细胞中清晰可见。我们检测到VASP从成人脑到第二孕期新皮质呈渐进性表达。因此，我们认为VASP可能在人类新生儿大脑皮质发育的调节中起关键作用。
