反硝化产碱菌天青蛋白编码基因的分离与测序：与铜绿假单胞菌和粪产碱菌中蓝色铜蛋白编码基因的比较
Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis.
作者信息
Hoitink C W, Woudt L P, Turenhout J C, van de Kamp M, Canters G W
机构信息
Gorlaeus Laboratories, Leiden University, The Netherlands.
出版信息
Gene. 1990 May 31;90(1):15-20. doi: 10.1016/0378-1119(90)90434-s.
The gene (azu) encoding azurin from Alcaligenes denitrificans has been cloned and sequenced. The gene codes for a pre-protein with a 19-aa signal peptide. Comparison with the sequences coding for the blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis reveals the presence of ntrA and fnr boxes in front of all three genes, instead of a regular [-10, -35]-promoter. In P. aeruginosa, the azu gene is terminated by a bidirectional terminator and flanked by open reading frames on the opposite strand.
已克隆并测序了来自反硝化产碱菌的编码天青蛋白的基因（azu）。该基因编码一种带有19个氨基酸信号肽的前体蛋白。与编码铜绿假单胞菌和粪产碱菌蓝色铜蛋白的序列进行比较发现，这三个基因前面均存在ntrA和fnr框，而不是常规的[-10，-35]启动子。在铜绿假单胞菌中，azu基因由双向终止子终止，并且在相反链上两侧均有开放阅读框。
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