Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
J Biosci Bioeng. 2011 Mar;111(3):370-6. doi: 10.1016/j.jbiosc.2010.11.007. Epub 2010 Dec 16.
As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.
由于动态结构变化对于某些类 RNA 分子的功能至关重要，因此开发无需化学修饰即可实时监测 RNA 结构变化的方法非常重要。基于反式作用 RNA 可作为靶 RNA 三维结构分析和控制的探针的先前报道，我们应用该方法监测 RNA 的时变结构变化。我们设计并进行了一项使用简单模型 RNA 复合物作为靶标的原理验证研究。使用两个反式作用 RNA 成功地监测了靶 RNA 的时变结构变化，这两个反式作用 RNA 与双分子靶 RNA 稳定形成三元复合物，并分别作为催化剂连接靶复合物的两个 RNA 片段。
