A domain responsible for the transformation suppressor activity in Krev-1 protein.
作者信息
Kitayama H, Matsuzaki T, Ikawa Y, Noda M
机构信息
Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki.
出版信息
Jpn J Cancer Res. 1990 May;81(5):445-8. doi: 10.1111/j.1349-7006.1990.tb02589.x.
Abstract
Krev-1 cDNA encodes a ras-related protein and exhibits an activity of inducing flat revertants at certain frequencies (2-5% of total transfectants) when introduced into a v-K-ras-transformed mouse NIH3T3 cell line, DT. To explore the functional organization of Krev-1 protein, we constructed a series of chimeric genes consisting of fragments of H-ras and Krev-1 cDNAs, and tested their biological activities in DT cells. The results indicated that the determinant for the transformation suppressor activity resides in the N-terminal one-third of the Krev-1 encoded polypeptide within which a highly conserved, putative effector-binding domain is present.
摘要
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