Krev-1蛋白中负责转化抑制活性的结构域。
A domain responsible for the transformation suppressor activity in Krev-1 protein.
作者信息
Kitayama H, Matsuzaki T, Ikawa Y, Noda M
机构信息
Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki.
出版信息
Jpn J Cancer Res. 1990 May;81(5):445-8. doi: 10.1111/j.1349-7006.1990.tb02589.x.
Abstract
Krev-1 cDNA encodes a ras-related protein and exhibits an activity of inducing flat revertants at certain frequencies (2-5% of total transfectants) when introduced into a v-K-ras-transformed mouse NIH3T3 cell line, DT. To explore the functional organization of Krev-1 protein, we constructed a series of chimeric genes consisting of fragments of H-ras and Krev-1 cDNAs, and tested their biological activities in DT cells. The results indicated that the determinant for the transformation suppressor activity resides in the N-terminal one-third of the Krev-1 encoded polypeptide within which a highly conserved, putative effector-binding domain is present.
摘要
Krev-1 cDNA编码一种与Ras相关的蛋白质，当将其导入v-K-ras转化的小鼠NIH3T3细胞系DT时，它会以一定频率（占总转染子的2%-5%）表现出诱导扁平回复子的活性。为了探究Krev-1蛋白的功能组织，我们构建了一系列由H-ras和Krev-1 cDNA片段组成的嵌合基因，并在DT细胞中测试了它们的生物学活性。结果表明，转化抑制活性的决定因素位于Krev-1编码多肽的N端三分之一区域内，该区域存在一个高度保守的假定效应器结合结构域。
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本文引用的文献
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