唾液中氨基酸组成在衰老过程中的变化。

Changes in salivary amino acid composition during aging.

机构信息

Division of Oral Diagnosis, Department of Diagnosis and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan.

出版信息

In Vivo. 2010 Nov-Dec;24(6):853-6.

PMID:21164043

Abstract

BACKGROUND

it has been suggested that the features of saliva reflect the physiological and psychological state of primates as well as subprimates, however, studies revealing the relationship between aging and the concentrations of salivary amino acids are limited. In order to better understand their physiological role, age-related changes of salivary amino acids were investigated.

MATERIALS AND METHODS

forty-five participants including 5 children [6.60 ± 1.67 (5-9) years old], 20 adults [46.55 ± 14.68 (23-64) years old), and 20 senior citizens [71.60 ± 4.27 (66-82) years old] took part in this study. Whole saliva (one sample per each person) was collected in the daytime (10:00-11:00 or 14:00-15:00). Salivary amino acids were recovered after deproteinization with 5% trichloroacetic acid and determined by an amino acid analyzer.

RESULTS

glycine was the most abundant amino acid in the saliva. Glycine and lysine levels increased significantly (p<0.05) with aging, regardless of gender difference. When the glycine and lysine levels were plotted, much higher correlation (p<0.001) was observed. On the other hand, there was no significant correlation between the salivary concentration of glutamic acid or histidine and age.

CONCLUSION

salivary amino acid levels may be regarded as markers of aging.

摘要

背景

有人认为唾液的特征反映了灵长类动物和次灵长类动物的生理和心理状态，然而，揭示衰老与唾液氨基酸浓度之间关系的研究是有限的。为了更好地了解它们的生理作用，研究了与年龄相关的唾液氨基酸变化。

材料和方法

共有 45 名参与者，包括 5 名儿童[6.60±1.67（5-9）岁]、20 名成年人[46.55±14.68（23-64）岁]和 20 名老年人[71.60±4.27（66-82）岁]参加了这项研究。每个人在白天（10:00-11:00 或 14:00-15:00）采集一次全唾液（每人一个样本）。用 5%三氯乙酸沉淀后回收唾液氨基酸，并通过氨基酸分析仪测定。