Department of Internal Medicine, Kinki Central Hospital, Hyogo, Japan.
J Perinatol. 2011 Jun;31(6):430-3. doi: 10.1038/jp.2010.144. Epub 2010 Dec 16.
As neonatal blood contains a high proportion of fetal hemoglobin (HbF), it is difficult to use high-performance liquid chromatography (HPLC) method, latex-immunoturbidimetry (LA) method and enzymatic methods, which determine hemoglobin A(1C) (HbA(1C)) in order to provide the glycemic control indicators of neonates. In this study, we evaluated glycated hemoglobin (GHb) and glycated albumin (GA) as appropriate indicators of the glycemic control in the neonatal period.
Umbilical cord blood samples collected during delivery were subjected to measurements of GHb (HPLC methods using two different instruments, LA method, enzymatic method and affinity method) and serum GA.
HbA(1C) levels determined by the HPLC method, the LA method and the enzymatic method were as low as <3.0% in all the cases. Although GHb determined by the affinity method was 3.6 ± 0.2%, this method may not measure accurately the values of glycated HbF plus glycated HbA. Serum GA was 9.4 ± 1.1%.
We speculate that serum GA, but not GHb, could be used as glycemic control indicators in neonates.
由于新生儿血液中含有较高比例的胎儿血红蛋白（HbF），因此难以使用高效液相色谱法（HPLC）、胶乳免疫比浊法（LA）和酶法等方法来确定血红蛋白 A1c（HbA1c），从而为新生儿提供血糖控制指标。本研究旨在评估糖化血红蛋白（GHb）和糖化白蛋白（GA）作为新生儿血糖控制的合适指标。
分娩时采集脐血样本，分别采用两种不同仪器的 HPLC 法、LA 法、酶法和亲和法检测 GHb 和血清 GA。
HPLC 法、LA 法和酶法测定的 HbA1c 水平均低至＜3.0%。尽管亲和法测定的 GHb 为 3.6±0.2%，但该方法可能无法准确测量糖化 HbF 与糖化 HbA 的联合值。血清 GA 为 9.4±1.1%。
我们推测血清 GA 而非 GHb 可作为新生儿血糖控制的指标。
