角域荧光寿命成像：一项组织样体模研究。

Angular domain fluorescence lifetime imaging: a tissue-like phantom study.

作者信息

Najiminaini Mohamadreza, Vasefi Fartash, Tichauer Kenneth M, Lee Ting-Yim, Kaminska Bozena, Carson Jeffrey J L

机构信息

Imaging Program, Lawson Health Research Institute, London, ON, Canada.

出版信息

Opt Express. 2010 Oct 25;18(22):23247-57. doi: 10.1364/OE.18.023247.

DOI:10.1364/OE.18.023247

PMID:21164666

Abstract

We describe a fluorescence lifetime imaging technique employing the collimation detection capabilities of an angular filter array (AFA). The AFA accepts minimally scattered photons emitted from fluorophores up to 2 mm deep within turbid media. The technique, referred to as Angular Domain Fluorescence Lifetime Imaging (ADFLI), is described and its performance evaluated in comparison to a conventional (lens and pinhole) system. Results from a tissue-mimicking phantom demonstrated that ADFLI provides better spatial resolution and image contrast for fluorescent probes at greater depths compared to a lens and pinhole system.

摘要

我们描述了一种利用角滤波器阵列（AFA）的准直检测能力的荧光寿命成像技术。AFA接收来自浑浊介质中深度达2毫米的荧光团发射的最少散射光子。该技术被称为角域荧光寿命成像（ADFLI），本文对其进行了描述，并与传统（透镜和针孔）系统相比评估了其性能。组织模拟体模的结果表明，与透镜和针孔系统相比，ADFLI在更大深度下为荧光探针提供了更好的空间分辨率和图像对比度。