Surplice I M, Griffiths P D, Green A, Leeming R J
Clinical Chemistry Department, Children's Hospital, Birmingham, UK.
J Inherit Metab Dis. 1990;13(2):169-77. doi: 10.1007/BF01799682.
We describe the automation, using a Cobas Bio centrifugal analyser, of a method for dihydropteridine reductase (DHPR) assay in eluates from dried blood spots. This is used as part of a routine screening service of neonates with hyperphenylalaninaemia in the United Kingdom. Automation reduced reagent volumes and analysis time by about 80% and improved the precision of the assay. Relating DHPR activity to haemoglobin concentration of the eluate further improved the precision of the assay and removed some differences in 'apparent' DHPR activity between samples from different countries and different age groups.
我们描述了使用Cobas Bio离心分析仪对干血斑洗脱液中二氢蝶啶还原酶（DHPR）检测方法的自动化过程。这一方法在英国作为高苯丙氨酸血症新生儿常规筛查服务的一部分使用。自动化使试剂用量和分析时间减少了约80%，并提高了检测的精密度。将DHPR活性与洗脱液的血红蛋白浓度相关联，进一步提高了检测的精密度，并消除了来自不同国家和不同年龄组样本之间“表观”DHPR活性的一些差异。
