Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627 Belo Horizonte, MG Brazil, 31270901, Brazil.
Oncol Rep. 2011 Feb;25(2):499-502. doi: 10.3892/or.2010.1094. Epub 2010 Dec 10.
WWOX is a tumour suppressor gene altered in various human neoplasms. Deletion of WWOX is associated with bone metabolic defects and development of osteosarcoma in mice. We hypothesized that alterations of this gene are associated with the development of benign and malignant mesenchymal bone related lesions of the jaws. We investigated WWOX mRNA by nested reverse transcription-PCR and direct sequencing and quantitative real-time PCR in two osteosarcoma, two fibrosarcoma, eight ossifying fibroma and two fibrous dysplasia fresh samples. Malignancy was associated with a decreased WWOX mRNA expression. Aberrant transcription pattern was found in five samples; however, the relative quantification (RQ) of the WWOX mRNA in such lesions was not different from those carrying only the wild-type. We provide new evidence of WWOX alterations in osteosarcomas and demonstrate for the first time alterations of this gene in fibrosarcomas as well as in ossifying fibromas of the jaws.
WWOX 是一种在多种人类肿瘤中发生改变的肿瘤抑制基因。WWOX 的缺失与小鼠的骨代谢缺陷和骨肉瘤的发生有关。我们假设该基因的改变与颌骨良性和恶性间充质骨相关病变的发生有关。我们通过巢式逆转录-PCR 和直接测序以及定量实时 PCR 研究了两个骨肉瘤、两个纤维肉瘤、八个骨化性纤维瘤和两个纤维结构不良新鲜样本中的 WWOX mRNA。恶性与 WWOX mRNA 表达减少有关。在五个样本中发现了异常的转录模式；然而，这些病变中的 WWOX mRNA 的相对定量 (RQ) 与仅携带野生型的样本没有差异。我们提供了 WWOX 在骨肉瘤中改变的新证据，并首次证明了该基因在纤维肉瘤以及颌骨骨化性纤维瘤中的改变。
