Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Biotechnol Lett. 2011 Apr;33(4):769-75. doi: 10.1007/s10529-010-0497-0. Epub 2010 Dec 17.
The plant-saprophytic basidiomycete, Coprinopsis cinerea, produces and secretes various cellulases during cellulose degradation as the main extracellular proteins. Although enzymatic characterization of such cellulases has been frequently reported, the mechanism of their secretion remains unclear. This study focused on myosins, actin-based motor proteins, involved in protein secretion in C. cinerea. During cultivation under cellulase-inducing condition, no cellulase activity was observed when the mycelia were treated with 2,3-butanedione 2-monoxime (BDM), a general inhibitor of myosin ATPase. Furthermore, BDM treatment disrupted the localization of the Golgi apparatus, but not that of the endoplasmic reticulum. Three genes encoding myosin-like proteins (CcMyo1, CcMyo2 and CcMyo5) were identified from the C. cinerea genome database. Transcription of these genes was promoted when the fungus was grown under cellulase-inducing condition.
植物腐生担子菌侧耳属（Coprinopsis cinerea）在纤维素降解过程中会产生和分泌各种纤维素酶作为主要的细胞外蛋白。虽然经常有报道对这些纤维素酶进行酶学特性分析，但它们的分泌机制仍不清楚。本研究关注的是肌球蛋白，一种肌动蛋白为基础的马达蛋白，参与侧耳属（Coprinopsis cinerea）中的蛋白质分泌。在纤维素酶诱导条件下培养时，当用肌球蛋白 ATP 酶的通用抑制剂 2,3-丁二酮 2-单肟（BDM）处理菌丝体时，没有观察到纤维素酶活性。此外，BDM 处理破坏了高尔基体的定位，但不影响内质网的定位。从侧耳属（Coprinopsis cinerea）基因组数据库中鉴定出三个编码肌球蛋白样蛋白（CcMyo1、CcMyo2 和 CcMyo5）的基因。当真菌在纤维素诱导条件下生长时，这些基因的转录被促进。
