Molecular Cell Biology Division, Kanagawa Cancer Center Research Institute, Nakao, Asahi-ku, Yokohama, Japan.
FEBS Lett. 2011 Jan 21;585(2):286-90. doi: 10.1016/j.febslet.2010.12.010. Epub 2010 Dec 15.
Invasion of MDA-MB-231 breast cancer cells into three-dimensional (3-D) type I-collagen matrices depends on TGF-α. We characterized the steps of invasion mediated by TGF-α. Cell migration, as observed by videomicroscopy, was effectively stimulated by collagen, suggesting that TGF-α may specifically participate in the invasion of a 3-D collagen matrix. We assessed the role of small GTPases of the Rho family in the invasion. Cdc42 was found to be necessary for invasion but dispensable for cell migration. These results suggest that TGF-α mediates invasion into 3-D collagen matrices by initiating the formation of protrusions into collagen, likely through activation of Cdc42.
MDA-MB-231 乳腺癌细胞侵入三维（3-D）I 型胶原基质取决于 TGF-α。我们描述了 TGF-α介导的侵袭步骤。视频显微镜观察到细胞迁移被胶原有效刺激，表明 TGF-α可能特异性参与 3-D 胶原基质的侵袭。我们评估了 Rho 家族小 GTPases 在侵袭中的作用。发现 Cdc42 对于侵袭是必需的，但对于细胞迁移是可有可无的。这些结果表明，TGF-α通过启动向胶原形成突起来介导侵袭进入 3-D 胶原基质，可能通过激活 Cdc42。
