更昔洛韦和青蒿琥酯对星形胶质细胞瘤细胞中人巨细胞病毒的抗病毒活性。
Antiviral activity of ganciclovir and artesunate towards human cytomegalovirus in astrocytoma cells.
机构信息
AP-HP, Hôpital Lariboisière, Service de Bactériologie-Virologie, Laboratoire Associé au Centre National de Référence du cytomégalovirus, 2 rue Ambroise Paré, 75475 Paris Cedex 10, France.
出版信息
Antiviral Res. 2011 Feb;89(2):186-8. doi: 10.1016/j.antiviral.2010.12.002. Epub 2010 Dec 15.
Antimalarial drug artesunate inhibits cytomegalovirus (HCMV) replication in human fibroblasts. Astrocytes, the major cell type of the brain, support cytomegalovirus (HCMV) replication. The aim of the study was to assess the antiviral activity of artesunate in astrocytoma cell line U373MG in comparison with ganciclovir. The antiviral concentration inhibiting by 50% (IC(50)) the synthesis of viral DNA was measured by real-time PCR in parallel in U373MG and MRC-5 cells. Reference HCMV strains susceptible and resistant to ganciclovir, and clinical isolates were tested. Ganciclovir and artesunate had similar activity in U373MG cells and MRC-5 fibroblasts. The artesunate IC(50)s in U373MG cells (1.5-2.25 μM) were at least 36-fold lower than the 50% cytotoxicity concentrations. Then, the anti-HCMV activity of artesunate was demonstrated in a cancer cell line.
青蒿琥酯抑制人成纤维细胞中巨细胞病毒（HCMV）的复制。星形胶质细胞是大脑的主要细胞类型，支持巨细胞病毒（HCMV）的复制。本研究的目的是评估青蒿琥酯在星形细胞瘤细胞系 U373MG 中的抗病毒活性，并与更昔洛韦进行比较。通过实时 PCR 在 U373MG 和 MRC-5 细胞中平行测量抗病毒浓度抑制病毒 DNA 合成的 50%（IC（50））。测试了对更昔洛韦敏感和耐药的参考 HCMV 株以及临床分离株。更昔洛韦和青蒿琥酯在 U373MG 细胞和 MRC-5 成纤维细胞中具有相似的活性。青蒿琥酯在 U373MG 细胞中的 IC（50）（1.5-2.25 μM）至少比 50%细胞毒性浓度低 36 倍。然后，在癌细胞系中证明了青蒿琥酯的抗 HCMV 活性。
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