Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
J Ethnopharmacol. 2011 Mar 24;134(2):251-8. doi: 10.1016/j.jep.2010.12.003. Epub 2010 Dec 15.
This present study was carried out to evaluate the anti-inflammatory and analgesic effects of 85% ethanol extract (EE) of Commiphora myrrha and its different fractions partitioned with petroleum ether extract (EPE), ethyl acetate extract (EEA), n-butanol extract (EBu), and the water extract (ECY). Moreover, the chemical constituents in EPE were analyzed and identified by UPLC-QTOF/MS/MS.
The anti-inflammatory activities were investigated by utilizing the paw edema mice induced by formalin. In addition, we determined the levels of PGE(2) in the edema paw. While the analgesic activity was examined against thermally and chemically induced nociceptive pain in mice, using the acetic acid and hot-plate test methods. The effects of the administration of dolantin or indomethacin were also studied for references. The components in EPE were analyzed by the ultra-performance liquid chromatography coupled with mass spectrum.
In the anti-inflammatory test, EE inhibited the development of paw swelling induced by formalin significantly. The pharmacological activities of the petroleum ether fraction (EPE) were stronger than the EE extract and other fractions at the dose of 100mg/kg, and furthermore significantly decreased the levels of inflammatory factor PGE(2) in the edema paw tissue at the fourth hour after formalin injection. It has been also shown that the ethanol extract (EE) significantly reduced acetic acid-induced writhing response in mice at the dose of 200mg/kg, and 100mg/kg. The petroleum ether fraction (EPE) showed significant analgesic activity in the model at the dose of 100mg/kg (p<0.01), and the ethyl acetate fraction (EEA) exhibited less analgesic activity (p<0.05). All test samples showed no significant analgesic activity on the hot plate pain threshold in mice. The UPLC-MS/MS chromatogram analysis of EPE stated that EPE contains the ingredients of sesquiterpenes, diterpenes, and diterpenic acids. Moreover, seven main compounds were identified.
These data demonstrated that the EE and EPE posses analgesic and anti-inflammatory activities and may support the fact the traditional application of this herb in treating various diseases associated with inflammatory pain.
本研究旨在评估 Commiphora myrrha 的 85%乙醇提取物（EE）及其用石油醚提取物（EPE）、乙酸乙酯提取物（EEA）、正丁醇提取物（EBu）和水提取物（ECY）分离的不同馏分的抗炎和镇痛作用。此外，通过 UPLC-QTOF/MS/MS 分析并鉴定了 EPE 中的化学成分。
利用甲醛诱导的爪肿胀小鼠研究抗炎活性。此外，我们还测定了肿胀爪中 PGE(2)的水平。同时，我们还使用醋酸和热板试验方法，检测了镇痛活性对小鼠热和化学诱导性疼痛的影响，并对杜冷丁和吲哚美辛的给药效果进行了研究。通过超高效液相色谱-质谱分析了 EPE 中的成分。
在抗炎试验中，EE 显著抑制甲醛诱导的爪肿胀发展。在 100mg/kg 剂量下，石油醚馏分（EPE）的药理活性强于 EE 提取物和其他馏分，并且在甲醛注射后第 4 小时显著降低了肿胀爪组织中炎症因子 PGE(2)的水平。结果还表明，乙醇提取物（EE）在 200mg/kg 剂量下显著减少了小鼠醋酸引起的扭体反应，在 100mg/kg 剂量下石油醚馏分（EPE）表现出显著的镇痛活性（p<0.01），乙酸乙酯馏分（EEA）表现出较弱的镇痛活性（p<0.05）。所有测试样品在小鼠热板疼痛阈值上均无显著镇痛活性。EPE 的 UPLC-MS/MS 色谱分析表明，EPE 含有倍半萜、二萜和二萜酸等成分。此外，鉴定了 7 种主要化合物。
这些数据表明，EE 和 EPE 具有镇痛和抗炎活性，可以支持该草药在治疗与炎症性疼痛相关的各种疾病方面的传统应用。
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