Department of Biology, St. Mary's College of Maryland, 18952 East Fisher Road, St. Mary's City, MD 20686, USA.
Dev Comp Immunol. 2011 May;35(5):521-4. doi: 10.1016/j.dci.2010.12.008. Epub 2010 Dec 17.
Caenorhabditis elegans has become a useful model for studying innate immunity. ELT-2, which is homologous to human GATA-4, -5 and -6, is considered the primary GATA transcription factor controlling intestinal immunity in C. elegans. In this study, we characterize the timeline of intestinal distension in nematodes where ELT-2 and another intestinal GATA transcription factor, ELT-7, are abrogated by RNAi using two different models: colonization and toxin-based infections by Pseudomonas aeruginosa. We show that both ELT-2 and ELT-7 are important for survival of C. elegans exposed to P. aeruginosa. Intestinal distension is accelerated in elt-2 RNAi nematodes, and is observed in colonization but not toxin-based Pseudomonas infection. Upon onset of intestinal distension, nematodes die within 24 h, regardless of experimental treatment. These data provide new insight into the role of ELT-2 and ELT-7 in protecting C. elegans against P. aeruginosa infection.
秀丽隐杆线虫已成为研究先天免疫的有用模型。ELT-2 与人 GATA-4、-5 和 -6 同源，被认为是控制秀丽隐杆线虫肠道免疫的主要 GATA 转录因子。在这项研究中，我们使用两种不同的模型（定植和基于毒素的铜绿假单胞菌感染）通过 RNAi 来描述 ELT-2 和另一种肠道 GATA 转录因子 ELT-7 缺失的线虫肠道扩张的时间进程。我们表明，ELT-2 和 ELT-7 对于暴露于铜绿假单胞菌的秀丽隐杆线虫的存活都是重要的。elt-2 RNAi 线虫的肠道扩张加速，在定植但不是基于毒素的铜绿假单胞菌感染中观察到。在肠道扩张开始时，线虫在 24 小时内死亡，无论实验处理如何。这些数据为 ELT-2 和 ELT-7 在保护秀丽隐杆线虫免受铜绿假单胞菌感染中的作用提供了新的见解。
