Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovského nám. 2, 162 06 Praha 6, Czech Republic.
J Synchrotron Radiat. 2011 Jan;18(1):29-30. doi: 10.1107/S0909049510030700. Epub 2010 Nov 5.
Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn(2+) ions was confirmed by X-ray fluorescence. First crystallographic data were obtained.
迄今用于攻击靶细胞核酸的抗癌药物基于动物或真菌核糖核酸酶。植物核酸酶的细胞毒性副作用已被证实有所降低。具有单链和双链 RNA 和 DNA 活性的番茄双功能核酸酶 1 作为重组蛋白在烟草叶片中产生。该酶在几种不同的结晶条件下结晶。通过 X 射线荧光证实存在 Zn(2+) 离子。获得了第一批晶体学数据。
