反相高效液相色谱法快速测定β-氨基异丁酸

Rapid determination of beta-aminoisobutyric acid by reversed-phase high-performance liquid chromatography.

作者信息

Ladrón de Guevara O, Cortinas de Nava C, Padilla P, Espinosa J, Cebrian M, García L

机构信息

Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico, D.F.

出版信息

J Chromatogr. 1990 Jun 8;528(1):35-41. doi: 10.1016/s0378-4347(00)82360-9.

DOI:10.1016/s0378-4347(00)82360-9

PMID:2117018

Abstract

For the determination of beta-aminoisobutyric acid (BAIBA) in urine samples in which the beta-alanine concentrations are higher than those of BAIBA, the resolution between these two amino acids, separated by reversed-phase liquid chromatography on an octadecylsilane column, was optimized. The chromatographic analysis included precolumn derivatization of amino acids with o-phthalaldehyde, followed by a 15-min isocratic elution and detection at 340 nm. Because of its simplicity, this method should be useful for monitoring urinary excretion of BAIBA.

摘要

对于尿液样本中β-丙氨酸浓度高于β-氨基异丁酸（BAIBA）的情况，优化了在十八烷基硅烷柱上通过反相液相色谱法分离这两种氨基酸时的分离度。色谱分析包括用邻苯二甲醛对氨基酸进行柱前衍生化，随后进行15分钟等度洗脱并在340nm处检测。由于其简便性，该方法对于监测BAIBA的尿排泄应该是有用的。