Mago N, Khuller G K
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
J Gen Microbiol. 1990 Jun;136(6):993-6. doi: 10.1099/00221287-136-6-993.
Lipids constituted around 5% of the dry weight in Candida albicans 3153, while sterols and phospholipids accounted for 1.2% and 1.1% respectively. Phospholipids were mainly localized in the microsomal fraction; phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were the major phospholipids. Incorporation studies with [14C]acetate and [32P]orthophosphoric acid demonstrated that PS was synthesized at the highest rate followed by PC, PE and PI. There was little difference in either the content of the rate of biosynthesis of PC, PE and PI. Incorporation of labelled serine, ethanolamine and choline revealed serine to be a precursor for PC, PE and PS, ethanolamine for PC and PE, and choline for PC biosynthesis only.
在白色念珠菌3153中，脂质约占干重的5%，而固醇和磷脂分别占1.2%和1.1%。磷脂主要定位于微粒体部分；磷脂酰丝氨酸（PS）、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）是主要的磷脂。用[14C]乙酸盐和[32P]正磷酸进行的掺入研究表明，PS的合成速率最高，其次是PC、PE和PI。PC、PE和PI的生物合成速率或含量几乎没有差异。掺入标记的丝氨酸、乙醇胺和胆碱表明，丝氨酸是PC、PE和PS的前体，乙醇胺是PC和PE的前体，胆碱仅是PC生物合成的前体。
