School of Science, Zhejiang Sci-Tech University, Hangzhou, China.
Biotechnol Lett. 2011 Apr;33(4):763-8. doi: 10.1007/s10529-010-0496-1. Epub 2010 Dec 18.
Growth and sporulation of Verticillium lecanii on inert and organic carriers (sugar-cane bagasse, corncob, rice straw, polyurethane foam and activated carbon) in a solid-state fermentation process was studied. Sugar-cane bagasse and polyurethane foam produced 10(10) spores g(-1) dry carrier whereas corncob, rice straw, and activated carbon yielded, respectively 8 × 10(9), 4 × 10(9), and 3 × 10(8) spores g(-1). Chitinase activity of the conidia was in the following order: sugar-cane bagasse (3.3 U mg(-1)) > wheat bran (3.0 U mg(-1)) > polyurethane foam (2.7 U mg(-1)). There was no significant difference (2.5-2.7 U mg(-1)) in the proteinase activity among the conidia from the three cultures. Scanning electron microscopy shows that aerial mycelium freely penetrated into the internal area of polyurethane foam. Sugar-cane bagasse provided enough area for vegetative hyphae to attach. Of the carriers analyzed, polyurethane foams and sugar-cane bagasse were the best carriers for V. lecanii growth and spore production.
研究了在固态发酵过程中，纤维枝孢（Verticillium lecanii）在惰性和有机载体（甘蔗渣、玉米芯、稻草、聚氨酯泡沫和活性炭）上的生长和孢子形成。甘蔗渣和聚氨酯泡沫每克干载体产生 10(10)个孢子，而玉米芯、稻草和活性炭分别产生 8×10(9)、4×10(9)和 3×10(8)个孢子。分生孢子的几丁质酶活性顺序为：甘蔗渣（3.3 U mg(-1)) > 麦麸（3.0 U mg(-1)) > 聚氨酯泡沫（2.7 U mg(-1))。三种培养物的分生孢子蛋白酶活性无显著差异（2.5-2.7 U mg(-1))。扫描电子显微镜显示，气生菌丝可自由穿透聚氨酯泡沫的内部区域。甘蔗渣为营养菌丝提供了足够的附着面积。在所分析的载体中，聚氨酯泡沫和甘蔗渣是最适合纤维枝孢生长和孢子生产的载体。
