Department of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart, Pfaffenwaldring 57, Stuttgart, Germany.
Arch Virol. 2011 Feb;156(2):347-52. doi: 10.1007/s00705-010-0876-9. Epub 2010 Dec 19.
Begomovirus is the largest genus within the family Geminiviridae and includes economically important plant DNA viruses infecting a broad range of plant species and causing devastating crop diseases, mainly in subtropical and tropical countries. Besides cultivated plants, many weeds act as virus reservoirs. Eight begomovirus isolates from Bolivian weeds were examined using rolling-circle amplification (RCA) and restriction fragment length polymorphism (RFLP). An efficient, novel cloning strategy using limited Sau3A digestion to obtain tandem-repeat inserts allowed the sequencing of the complete genomes. The viruses were classified by phylogenetic analysis as typical bipartite New World begomoviruses. Four of them represented distinct new virus species, for which the names Solanum mosaic Bolivia virus, Sida mosaic Bolivia virus 1, Sida mosaic Bolivia virus 2, and Abutilon mosaic Bolivia virus are proposed. Three were variants of a new strain of Sida micrantha mosaic virus (SimMV), SimMV-rho[BoVi07], SimMV-rho[Bo:CF1:07] and SimMV-rho[Bo:CF2:07], and one was a new variant of a previously described SimMV, SimMV-MGS2:07-Bo.
双生病毒科是最大的病毒属，包括经济上重要的感染广泛植物物种并引起严重作物病害的植物 DNA 病毒，主要在亚热带和热带国家。除了栽培植物外，许多杂草是病毒的储存库。使用滚环扩增 (RCA) 和限制性片段长度多态性 (RFLP) 检查了来自玻利维亚杂草的 8 个双生病毒分离物。使用有限的 Sau3A 消化获得串联重复插入的有效新型克隆策略允许对完整基因组进行测序。通过系统发育分析，这些病毒被分类为典型的二分体新世界双生病毒。其中 4 种代表了独特的新病毒种，为此建议使用以下名称：Solanum mosaic Bolivia virus、Sida mosaic Bolivia virus 1、Sida mosaic Bolivia virus 2 和 Abutilon mosaic Bolivia virus。其中 3 种是新的 Sida micrantha mosaic virus (SimMV) 菌株的变体，SimMV-rho[BoVi07]、SimMV-rho[Bo:CF1:07]和 SimMV-rho[Bo:CF2:07]，而另一种是先前描述的 SimMV 的新变体，SimMV-MGS2:07-Bo。
