高效液相色谱-蒸发光散射检测法测定番茄中番茄苷A
[Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection].
作者信息
Lu Fenglai, Liu Jinlei, Chen Yueyuan, Wu Jianzhang, Li Dianpeng
机构信息
Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, China.
出版信息
Se Pu. 2010 Sep;28(9):908-11.
A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61-3.05 mg with the correlation coefficient of 0. 999 5. The average recoveries were 97.9%-104.8% with the relative standard deviations (RSD) < or = 4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.
建立了一种用高效液相色谱（HPLC）-蒸发光散射检测（ELSD）法测定番茄（Lycopersicon esculentum MILL）中番茄苷A的方法。结果表明，番茄苷A在0.61 - 3.05 mg范围内线性关系良好，相关系数为0.999 5。平均回收率为97.9% - 104.8%，相对标准偏差（RSD）≤4.14%（n = 5）。该方法简便、灵敏，适用于番茄苷A的分析及番茄原料和提取物的质量控制。
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