Ji Shu-mei, Hu Sheng-ai, He Rui-rong
Department of Physiology, Hebei Medical University, Shijazhuang 050017, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 May;21(2):146-9.
Using double immunohistochemical method for Fos and tyrosine hydroxylase(TH) to examine the effects of intracerebroventricular (icv) administration of adrenomedullin (AM) on catecholaminergic neurons and the expression of c-fos gene in rat brain nuclei involved in cardiovascular regulation in order to define whether the effects of central administration of adrenomedullin (AM) were induced by activating the catecholaminergic neurons.
(1) Following icy administration of AM (3 nmol/kg), Fos-like immunoreactivity neurons were markedly increased in several brain areas of the rat, including the brainstem, the hypothalamus and the forebrain. (2) Following icy administration of AM (3 nmol/kg), double-labeled neurons for Fos and TH increased significantly in the area postrema (AP), the nucleus of the solitary tract (NTS), the nucleus paragigantocellularis lateralis (PGL) and the locus coeruleus (LC). (3) Pretreatment with calcitonin gene-related peptide receptor antagonism CGRP (8-37) (30 nmol/kg) significantly reduced the action of AM (3 nmol/kg) in the brain.
AM activates the nuclei involved in cardiovascular regulation in the forebrain, the hypothalamus and the brainstem, and that the central actions of AM are induced by activating the catecholaminergic neurons of brainstem nuclei involved in cardiovascular regulation. CGRP receptor can mediate the effects of AM in brain.
采用Fos与酪氨酸羟化酶（TH）双重免疫组化方法，研究脑室注射肾上腺髓质素（AM）对大鼠脑内参与心血管调节的核团中儿茶酚胺能神经元及c-fos基因表达的影响，以确定中枢给予肾上腺髓质素（AM）的作用是否由激活儿茶酚胺能神经元所诱导。
（1）脑室注射AM（3 nmol/kg）后，大鼠的几个脑区，包括脑干、下丘脑和前脑，Fos样免疫反应性神经元明显增加。（2）脑室注射AM（3 nmol/kg）后，最后区（AP）、孤束核（NTS）、外侧巨细胞旁核（PGL）和蓝斑（LC）中Fos与TH的双标记神经元显著增加。（3）用降钙素基因相关肽受体拮抗剂CGRP（8-37）（30 nmol/kg）预处理可显著降低AM（3 nmol/kg）在脑中的作用。
AM激活前脑、下丘脑和脑干中参与心血管调节的核团，且AM的中枢作用是通过激活参与心血管调节的脑干核团中的儿茶酚胺能神经元所诱导。CGRP受体可介导AM在脑中的作用。
