Zheng Hui-li, Ni Jiang, Bian Shu-ling
Department of physiology, Harbin Medical University, Harbin 150086, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 May;21(2):227-30.
The expression of VEGF in rat ovaries corpus luteum and its expression pattern were observed to investigate the effect of VEGF on luteal formation and regression.
The model of immature rat of pseudopregnant was established using pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), the expression of VEGF in corpus luteum was detected by immunohistochemistry, the levels of VEGF in corpus luteum was measured by ELISA, and the levels of NO in corpus luteum was measured by chemistry assay.
VEGF expressed weakly in rat corpus luteum on the day 1, and enhanced gradually from day 3 to day 5, then went up to the peak on the day 7, and maintained to day 9. On the day 11, the expression of VEGF began to decrease. The levels of VEGF were similar to the expression of VEGF. The levels of NO appeared like double wave. The levels increased gradually from day 1 to day 5, and peaked on the day 7, then decreased on the day 9, while lightly increased on the day 11, and showed significant increase and reached the highest on the day 13, then decreased the lowest on the day 15.
There is a intimate temporal relationship between the expression of VEGF and angiogenesis in corpus luteum, VEGF may play a role in luteum formation by improving angiogenesis mediated by NO, NO may play a role in luteum regression as a luteolytic during the late luteal phase.
观察血管内皮生长因子（VEGF）在大鼠卵巢黄体中的表达及其表达模式，以探讨VEGF对黄体形成和退化的影响。
采用孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）建立未成熟假孕大鼠模型，用免疫组织化学法检测黄体中VEGF的表达，用酶联免疫吸附测定法（ELISA）检测黄体中VEGF的水平，用化学分析法检测黄体中一氧化氮（NO）的水平。
VEGF在大鼠黄体第1天表达较弱，从第3天到第5天逐渐增强，然后在第7天达到峰值，并维持到第9天。在第11天，VEGF的表达开始下降。VEGF的水平与VEGF的表达相似。NO的水平呈双波状。从第1天到第5天逐渐升高，在第7天达到峰值，然后在第9天下降，而在第11天略有升高，并在第13天显著升高并达到最高，然后在第15天下降到最低。
VEGF的表达与黄体血管生成之间存在密切的时间关系，VEGF可能通过改善由NO介导的血管生成在黄体形成中发挥作用，NO可能在黄体后期作为黄体溶解因子在黄体退化中发挥作用。
