Shao Guo, Zhou Wei-Hua, Gao Cui-Ying, Zhang Ran, Lu Guo-Wei
Department of Neurobiology, Capital University of Medical Sciences, 100054 Beijing, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Feb;23(1):1-4.
To observe change of binding activity of HIF-1 with erythropoietin (EPO) hypoxia response element (HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning.
The hippocampus was removed from mice exposed to hypoxia for 0 run (control group), 1 run (H1 group) and 4 runs(H4 group). Electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO.
Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4.
The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.
观察低氧预处理小鼠海马中缺氧诱导因子-1（HIF-1）与促红细胞生成素（EPO）缺氧反应元件（HRE）结合活性的变化，并探讨这些变化与预处理之间的关系。
取暴露于低氧0次（对照组）、1次（H1组）和4次（H4组）的小鼠海马。采用电泳迁移率变动分析（EMSA）、染色质免疫沉淀（ChIP）和实时定量PCR检测HIF-1对EPO的HRE活性变化。
体外和体内结合试验均显示，H1组HIF-1与DNA的结合活性增加，H4组显著增加。
HIF-1与EPO的HRE结合活性增加被认为与低氧预处理有关。
