[葛根素对培养的大鼠大脑皮质星形胶质细胞的保护作用]
[The protective effect of puerarin on cultured rat cerebral cortical astrocytes].
作者信息
Dong L P, Han M, Yuan F, Xu L X, Wang T Y
机构信息
Beijing Neurosurgical Institute, Beijing 100050, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2001 May;17(2):141-3.
AIM
To study the effects of puerarin (Pue) on impairment of rat astrocytes in primary cell culture induced by oxygen-glucose deprivation (OGD) or sodium glutamate (Glu).
METHODS
Astrocyte damage induced by (OGD), Glu or (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD), as well as the action of Pue was measured by determining the intracellular water space (as measured by 3-O-methyl-[1- 3H]D-glucose uptake) of astrocytes and the leakage of lactate dehydrogenase (LDH) from astrocytes.
RESULTS
Following the exposure to OGD for 5 h, 0.5 mmol/L Glu or 1 mmol/L trans-ACPD for 1 h, the astrocyte volume and LDH leakage from astrocytes were increased. 0.1 mmol/L Pue, when co-incubated with OGD, Glu or trans-ACPD, reduced astrocytic swelling and the LDH leakage.
CONCLUSION
Pue had protective effects on astrocytes damaged by OGD, Glu or trans-ACPD.
目的
研究葛根素（Pue）对氧糖剥夺（OGD）或谷氨酸钠（Glu）诱导的原代细胞培养大鼠星形胶质细胞损伤的影响。
方法
通过测定星形胶质细胞的细胞内水空间（通过3 - O - 甲基 - [1 - 3H]D - 葡萄糖摄取来测量）和星形胶质细胞中乳酸脱氢酶（LDH）的泄漏，来检测由OGD、Glu或（±）-1 - 氨基环戊烷 - 反式 - 1,3 - 二羧酸（反式 - ACPD）诱导的星形胶质细胞损伤以及Pue的作用。
结果
在暴露于5小时的OGD、0.5 mmol/L Glu或1 mmol/L反式 - ACPD 1小时后，星形胶质细胞体积和星形胶质细胞中LDH泄漏增加。当与OGD、Glu或反式 - ACPD共同孵育时，0.1 mmol/L Pue可减轻星形胶质细胞肿胀和LDH泄漏。
结论
Pue对由OGD、Glu或反式 - ACPD损伤的星形胶质细胞具有保护作用。
Zotero 插件