Schäfer K P, Voss T, Melchers K, Eistetter H
Byk Gulden Pharmaceuticals, Department of Molecular Biology, Konstanz, Federal Republic of Germany.
Lung. 1990;168 Suppl:851-9. doi: 10.1007/BF02718219.
The genes for all three of the bona fide surfactant associated proteins have been cloned, allowing their production by recombinant DNA technology. In addition, improved protocols for the isolation of the natural surfactant proteins (NSP) made them available in larger quantities. Whereas, the NSP are often mixtures of allelic variants or functional isomers from gene families, the recombinant proteins (RSP) are obtained as single pure protein species. Antibodies directed against the N/RSP in combination with DNA probes have allowed new approaches to analyze the formation, location, transport, structure and functional capacities of these molecules as well as their interactions with one another and the phospholipids.
所有三种真正的表面活性剂相关蛋白的基因均已被克隆，这使得通过重组DNA技术生产这些蛋白成为可能。此外，改进后的天然表面活性剂蛋白（NSP）分离方案使其产量得以增加。鉴于NSP通常是基因家族中等位基因变体或功能异构体的混合物，而重组蛋白（RSP）则是单一的纯蛋白种类。针对N/RSP的抗体与DNA探针相结合，为分析这些分子的形成、定位、运输、结构和功能能力，以及它们彼此之间和与磷脂的相互作用提供了新方法。
