一种单重多重聚合酶链锁反应和 SNaPshot 微测序反应可对 42 个单核苷酸多态性进行分类，以将混合人群归入线粒体 DNA 单倍群。
A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups.
机构信息
Faculdade de Ciências Farmacêuticas, Unesp-Univ Estadual Paulista, Araraquara, SP, Brazil.
出版信息
Mitochondrion. 2011 Mar;11(2):296-302. doi: 10.1016/j.mito.2010.12.010. Epub 2010 Dec 21.
SNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations.
SNaPshot 微测序反应因其能够在单次测定中快速检测多种多态性而得到越来越多的应用。在这项工作中，我们描述了一种高度敏感的单核苷酸多态性 (SNP) 分型方法，可在单个 PCR 和 SNaPshot 多重反应中检测 42 个线粒体 DNA (mtDNA) SNP，以允许在拉丁美洲混合人群中进行单倍群分类。我们验证了该面板对 160 名巴西个体的分型。从 10pg 总 DNA 中获得了完整的 SNP 图谱。我们得出结论，在单个多重 PCR 和 SNaPshot 反应中构建和分型四十多个 mtDNA SNP 是可能的，具有敏感性和可靠性，可解决混合人群中的单倍群分类。
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