Institute of Legal Medicine, Münster University, Germany.
Forensic Sci Int Genet. 2011 Jun;5(3):216-21. doi: 10.1016/j.fsigen.2010.01.015. Epub 2010 Feb 18.
In this study six forensic cases are presented where the routine analysis of samples for short tandem repeats (STRs) failed. The sequencing of the mitochondrial hypervariable region I (HVR I) also failed. Nevertheless, it was possible to analyse the samples with mitochondrial DNA (mtDNA) single nucleotide polymorphisms (SNPs) via SNaPshot technique. The age of the analysed samples ranged from 2 months to 1400 years. Saliva-, blood-, sperm-, hair-, tooth- and bone-samples were investigated. Furthermore the mtDNA SNP analysis of a forensic case sample showing a mixed stain profile is presented. It was possible to discriminate two different haplogroups in this mixed-person stain. If compared to another mtDNA SNP profile that was found in a hair, the discriminating SNPs of the hair were as well found in the mixed-person stain. To disburden the SNP analysis in forensic casework, haplogroup assignment criteria and quality criteria for mtDNA SNaPshot analysis are announced.
本研究介绍了六个法医案例，其中常规短串联重复序列（STR）分析和线粒体高变区 I（HVR I）测序均失败。然而，通过 SNaPshot 技术对线粒体 DNA（mtDNA）单核苷酸多态性（SNP）进行分析成为可能。所分析样本的年龄从 2 个月到 1400 年不等，涉及唾液、血液、精液、毛发、牙齿和骨骼样本。此外，还介绍了一个显示混合污渍特征的法医案例样本的 mtDNA SNP 分析。在这种混合个人污渍中，可以区分出两个不同的单倍群。与在毛发中发现的另一个 mtDNA SNP 图谱相比，毛发中发现的区分 SNP 也存在于混合个人污渍中。为了减轻法医工作中的 SNP 分析负担，宣布了单倍群分配标准和 mtDNA SNaPshot 分析的质量标准。
