Department of Dental Hygiene, National Chung Hsing University, Taichung 402, Taiwan.
J Dent Res. 2011 Feb;90(2):168-74. doi: 10.1177/0022034510384619. Epub 2010 Dec 20.
Phytochemicals have been used as potential chemopreventive or chemotherapeutic agents. However, there are data suggesting a mutagenic effect of some phytochemicals. We hypothesized that safrole would have anticancer effects on human oral squamous cell carcinoma HSC-3 cells. Safrole decreased the percentage of viable HSC-3 cells via induction of apoptosis by an increased level of cytosolic Ca(2+) and a reduction in the mitochondrial membrane potential (ΔΨ(m)). Changes in the membrane potential were associated with changes in the Bax, release of cytochrome c from mitochondria, and activation of downstream caspases-9 and -3, resulting in apoptotic cell death. In vivo studies also showed that safrole reduced the size and volume of an HSC-3 solid tumor on a xenograft athymic nu/nu mouse model. Western blotting and flow cytometric analysis studies confirmed that safrole-mediated apoptotic cell death of HSC-3 cells is regulated by cytosolic Ca(2+) and by mitochondria- and Fas-dependent pathways.
植物化学物质已被用作潜在的化学预防或化学治疗剂。然而，有数据表明一些植物化学物质具有致突变作用。我们假设黄樟素对人口腔鳞状细胞癌细胞 HSC-3 具有抗癌作用。黄樟素通过增加细胞浆 Ca(2+)水平和降低线粒体膜电位（ΔΨ(m)）诱导细胞凋亡，从而降低 HSC-3 细胞的存活率。膜电位的变化与 Bax 的变化、细胞色素 c 从线粒体中的释放以及下游半胱天冬酶-9 和 -3 的激活有关，导致细胞凋亡死亡。体内研究还表明，黄樟素可减小异种移植裸鼠模型中 HSC-3 实体瘤的大小和体积。Western 印迹和流式细胞术分析研究证实，黄樟素介导的 HSC-3 细胞凋亡细胞死亡受细胞浆 Ca(2+)和线粒体及 Fas 依赖性途径调节。
