Seol Hyun-Joo, Oh Min-Jeong, Kim Hai-Joong
Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University, Seoul, Korea.
Hypertens Pregnancy. 2011;30(3):295-301. doi: 10.3109/10641950903115053. Epub 2010 Dec 21.
This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types.
We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells.
In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture.
VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.
本研究调查了血管内皮生长因子（VEGF）在两种不同血管壁细胞类型中诱导的内皮素-1（ET-1）生成情况。
我们在人脐静脉内皮细胞和主动脉平滑肌细胞中，使用实时聚合酶链反应和酶联免疫吸附测定法，分析了内皮素转换酶-1（ECE-1）抑制剂和基质金属蛋白酶-2（TIMP-2）组织抑制剂对VEGF诱导的ET-1表达的影响。
在人脐静脉内皮细胞中，ECE-1抑制剂磷酰胺素和TIMP-2均降低了VEGF诱导的ET-1生成。在主动脉平滑肌细胞中，TIMP-2抑制了VEGF诱导的ET-1生成，但磷酰胺素对培养物中的ET-1浓度没有影响。
VEGF诱导的ET-1生成在内皮细胞中可能依赖MMP-2或ECE-1；然而，在平滑肌细胞中，ET-1表达似乎仅由MMP-2诱导。
