[桃红四物汤抗血小板活化作用及其部分机制研究]
[Studies on anti-platelet activation effect and partial mechanisms of Taohong Siwu decoction].
作者信息
Han Lan, Peng Daiyin, Xu Fan, Wang Ning, Liu Qingyun, Dai Min, Liu Dong
机构信息
Key Laboratory of Chinese Medicine Research and Development in Anhui Province, Anhui College of Traditional Chinese Medicine, Hefei 230038, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(19):2609-12.
OBJECTIVE
To explore the anti-platelet activation effect and partial mechanisms of Taohong Siwu decoction (TSD).
METHOD
The effect to venous thrombosis model and pulmonary thromboembolism model induced by vein injecting ADP and Adr was observed. The platelet adhesion rate was analyzed by using spinning glass bottle, and the platelet aggregation rate induced by ADP, Adr was analyzed by using turbidimetry. The acute blood stasis rat model was established to analyze the content of plasm TXB2 and PGI2 by RIA, and the content of VWF, GMP-14 by ELISA.
RESULT
TSD could effectively reduce platelet the adhesion rate of normal rat, inhibit the platelet aggregation of normal rat induced by ADP, Adr. It significantly reduced the plasma TXB2 VWF, and GMP-140 level of blood stasis rats. It also had significant tendency to increase 6-keto-PGF1alpha level.
CONCLUSION
TSD possessed obvious activity of inhibiting platelet activation. The mechanism related with the restraining of platelet adhesion, platelet aggregation and platelet releasion.
目的
探讨桃红四物汤（TSD）抗血小板活化作用及部分机制。
方法
观察其对静脉注射ADP和Adr诱导的静脉血栓模型和肺血栓栓塞模型的影响。采用旋转玻璃瓶法分析血小板黏附率，用比浊法分析ADP、Adr诱导的血小板聚集率。建立急性血瘀大鼠模型，用放射免疫分析法（RIA）分析血浆TXB2和PGI2含量，用酶联免疫吸附测定法（ELISA）分析VWF、GMP-14含量。
结果
TSD能有效降低正常大鼠血小板黏附率，抑制ADP、Adr诱导的正常大鼠血小板聚集。显著降低血瘀大鼠血浆TXB2、VWF和GMP-140水平。对升高6-酮-PGF1α水平也有明显趋势。
结论
TSD具有明显抑制血小板活化的活性。其机制与抑制血小板黏附、聚集及释放有关。
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