School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, PR China.
J Ethnopharmacol. 2011 Jan 27;133(2):796-802. doi: 10.1016/j.jep.2010.11.008. Epub 2010 Nov 10.
"Dragon's Blood" has been used as a medicine since ancient times by many cultures. In traditional Chinese medicine, the resin obtained from Daemonorops draco (RDD) and the resin from Dracaena cochinchinensis (RDC) are equally prescribed as "Dragon's Blood" for facilitating blood circulation.
To verify the traditional efficacy and elucidate the mechanism, the present study compared the chemical profiles and the pharmacological effects of two species of "Dragon's Blood" mainly used in China.
A UPLC-MS fingerprinting method was developed to compare the chemical profiles of the two medicines. The anti-platelet aggregation effects of the two medicines induced by arachidonic acid (AA) were investigated.
The chemical profiles of these two species of "Dragon's Blood" were significantly different. The characteristic constituents were found to be: flavanes in RDD and stilbenes in RDC. In the in vivo platelet inhibition test, performed with the dose of 200 mg/kg on rats, the peak inhibitory effects of RDD and RDC were 35.8% and 27.6%, respectively, compared with the control group. With the in vitro concentrations of 0.2, 0.4 and 0.8 mg/ml, RDD exerted significant inhibition of aggregation by 18.7%, 20.0%, and 61.6%, respectively, and RDC exerted significant inhibition of aggregation by 13.3%, 20.2%, and 31.6%, respectively.
The fingerprinting method used here is suitable for distinguishing them. All pharmacological tests indicated that RDD was more potent than RDC against platelet aggregation.
自古以来，许多文化都将“龙血”用作药物。在中国传统医学中，从龙舌兰（RDD）和海南龙血树（RDC）中获得的树脂都被同等地用作“龙血”，以促进血液循环。
为了验证传统功效并阐明其机制，本研究比较了两种在中国主要使用的“龙血”的化学特征和药理作用。
建立了一种 UPLC-MS 指纹图谱法来比较这两种药物的化学特征。研究了两种药物对花生四烯酸（AA）诱导的血小板聚集的抑制作用。
这两种“龙血”的化学特征有明显差异。特征成分分别为 RDD 中的黄烷和 RDC 中的芪类。在体内血小板抑制试验中，用 200mg/kg 剂量对大鼠进行测试，RDD 和 RDC 的最大抑制作用分别为 35.8%和 27.6%，与对照组相比。在 0.2、0.4 和 0.8mg/ml 的体外浓度下，RDD 对聚集的抑制作用分别为 18.7%、20.0%和 61.6%，RDC 对聚集的抑制作用分别为 13.3%、20.2%和 31.6%。
这里使用的指纹图谱法适合区分它们。所有药理试验表明，RDD 对血小板聚集的抑制作用强于 RDC。
