Knibiehler B, Mirre C, Le Parco Y
Biologie de la Différenciation Cellulaire, Faculté des Sciences de Luminy, Marseille, France.
Cell Differ Dev. 1990 May;30(2):147-57. doi: 10.1016/0922-3371(90)90082-8.
We have developed and characterized a battery of specific polyclonal antibodies directed against specific portions of the alpha-chain of collagen type IV synthesized in Drosophila by the gene DCg1. Here, we describe the use of these antibodies together with in situ hybridization experiments in an attempt to study the expression and localization of collagen type IV during Drosophila oogenesis and early embryogenesis. The results clearly demonstrate that DCg1 is maternally expressed by follicle cells and that the collagen type IV chain produced is stockpiled in the growing oocyte. During the gastrulation stages, this component of Drosophila basement membranes concentrated on cells involved in the gradual invaginations leading to morphogenetic furrows. The presence of collagen type IV, which is an RGD-bearing molecule, during early stages of Drosophila development is discussed in comparison with the crucial, active role its vertebrate counterpart is supposed to play in morphogenetic processes.
我们已经开发并鉴定了一系列针对果蝇中由基因DCg1合成的IV型胶原蛋白α链特定部分的特异性多克隆抗体。在此，我们描述了这些抗体与原位杂交实验一起的使用，试图研究果蝇卵子发生和早期胚胎发育过程中IV型胶原蛋白的表达和定位。结果清楚地表明，DCg1由卵泡细胞母体表达，并且产生的IV型胶原蛋白链储存在生长中的卵母细胞中。在原肠胚形成阶段，果蝇基底膜的这一成分集中在参与导致形态发生沟的逐渐内陷的细胞上。与脊椎动物对应物在形态发生过程中所起的关键、积极作用相比，讨论了果蝇发育早期含RGD分子的IV型胶原蛋白的存在情况。
