Yang Yan-ling, Wang Hong-xin, Yang Yu-hong
Department of Pharmacology, Jinzhou Medical College, Jinzhou 121001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2002 Nov;18(4):358-61.
In order to study the effects of kappa-opioid receptor activation, we used cultured cardiomyocytes to study the inhibitory effects of U50,488H on cellular proliferation, protein content in the presence or absence of nor-binaltorphimine (nor-BNI).
The cellular proliferation was determined with crystal violet staining and the protein content was assayed with Lowry's method.
A kappa-opioid receptor agonist U50,488H at 0.1 micromol/L-10 micromol/L inhibited the cellular proliferation and protein content of cultured myocardial cells in a dose-dependent manner. The inhibitory effects of U50,488H were completely blocked by pretreatment with nor-BNI, a specific kappa-opioid receptor antagonist at 1 micromol/L.
U50,488H inhibited the cultured myocardial cells' growth. The inhibitory effects of U50,488H are involved in mediating the action of kappa-opioid receptor stimulation.
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