用于用RNA病毒的cDNA克隆对植物进行转染的简单高效的生物弹道程序。
Simple and efficient biolistic procedure for the plant transfection with cDNA clones of RNA viruses.
作者信息
Predajňa L, Nagyová A, Subr Z
机构信息
Slovak Academy of Sciences, Bratislava, Slovak Republic.
出版信息
Acta Virol. 2010;54(4):303-6. doi: 10.4149/av_2010_04_303.
Simple, fast, low-cost, and efficient procedure for DNA delivery to the cell nuclei of whole plants was developed. The procedure was optimized for the Plum pox virus (PPV) and its host Nicotiana benthamiana. It is based on the leaf bombardment with tungsten microparticles with bound DNA using common air gun. The procedure did not require special equipment and source of driving gas. The transfection efficiency obtained by the newly developed procedure was close to 100%, but this level dropped with the age of bombarded plants.
开发了一种简单、快速、低成本且高效的将DNA导入整株植物细胞核的方法。该方法针对李痘病毒（PPV）及其宿主本氏烟草进行了优化。它基于使用普通气枪用结合了DNA的钨微粒轰击叶片。该方法不需要特殊设备和驱动气体源。新开发的方法获得的转染效率接近100%，但随着被轰击植物年龄的增长，这一水平会下降。
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