Ras p21与蛋白激酶A和C在刺激NIH/3T3细胞基因表达中的作用方式比较。
Comparison of the mode of action of ras p21 with those of protein kinases A and C in the stimulation of gene expression in NIH/3T3 cells.
作者信息
Oku N, Kaibuchi K, Fukumoto Y, Hori Y, Fujioka H, Takai Y
机构信息
Department of Biochemistry, Kobe University School of Medicine, Japan.
出版信息
FEBS Lett. 1990 Aug 20;269(1):148-52. doi: 10.1016/0014-5793(90)81141-a.
To compare the mode of action of ras p21 with those of protein kinases A and C in the regulation of gene expression in NIH/3T3 cells, we investigated the transcriptional activity of various enhancer/promoters and enhancer motifs in the cells transfected with the c-Ha-rasva112 complementary DNA (cDNA). The results indicate that the c-Ha-rasva112 protein stimulates the enhancer/promoters of the c-fos gene, the metallothionein IIA gene, the simian virus 40 (SV40) virus genome and the Rous sarcoma (RS) virus genome, and the serum-response element and the 12-O-tetradecanoylphorbol-13-acetate (TPA)-response element in a manner independent of protein kinases A and C in NIH/3T3 cells.
为了比较Ras p21与蛋白激酶A和C在调控NIH/3T3细胞基因表达中的作用方式，我们研究了用c-Ha-rasva112互补DNA（cDNA）转染的细胞中各种增强子/启动子和增强子基序的转录活性。结果表明，在NIH/3T3细胞中，c-Ha-rasva112蛋白以独立于蛋白激酶A和C的方式刺激c-fos基因、金属硫蛋白IIA基因、猿猴病毒40（SV40）病毒基因组和劳斯肉瘤（RS）病毒基因组的增强子/启动子，以及血清反应元件和12-O-十四烷酰佛波醇-13-乙酸酯（TPA）反应元件。
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