Trubetskoy V S, Koshkina N V, Omel'yanenko V G, L'vov V L, Dmitriev B A, Petrov A B, Torchilin V P
USSR Cardiology Research Center, Moscow.
FEBS Lett. 1990 Aug 20;269(1):79-82. doi: 10.1016/0014-5793(90)81123-6.
FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actinomycin D-sensitized mice. Fluorescence intensity increase was observed upon the insertion of FITC-LPS into the membrane of dehydration/rehydration vesicles and vesicles obtained by co-sonication of lipid suspension and FITC-LPS. Following FITC-LPS fluorescence polarization it was shown that the substance seems to be clusterized in the liposomal membrane starting from FITC-LPS/lipid molar ratio 1:800.
来自脑膜炎奈瑟菌的异硫氰酸荧光素（FITC）标记的脂多糖（LPS）可作为一种探针，用于追踪LPS掺入脂质体膜的过程，并研究其与双层膜的相互作用。通过物理化学方法以及放线菌素D致敏小鼠中脂质体LPS毒性降低，证明了FITC-LPS掺入双层膜。当FITC-LPS插入脱水/再水化囊泡以及通过脂质悬浮液与FITC-LPS共超声处理获得的囊泡膜中时，观察到荧光强度增加。追踪FITC-LPS荧光偏振显示，从FITC-LPS/脂质摩尔比1:800开始，该物质似乎在脂质体膜中聚集。
