低温电子断层扫描技术：方法学、进展和生物学应用。
Cryo-electron tomography: methodology, developments and biological applications.
机构信息
Max Planck Institute of Biochemistry, Martinsried, Germany.
出版信息
J Microsc. 2011 Jun;242(3):221-7. doi: 10.1111/j.1365-2818.2010.03478.x. Epub 2010 Dec 23.
Abstract
Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
摘要
冷冻电子断层成像技术可实现对处于分子分辨率水平的冷冻水合、玻璃化生物材料的三维可视化。本文总结了最重要的样品制备方法和相关技术要点，以及该技术在从分离的大分子复合物到整个细胞和组织的最新生物学应用。
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