Department of Chemical Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
Lett Appl Microbiol. 2011 Feb;52(2):116-22. doi: 10.1111/j.1472-765X.2010.02974.x. Epub 2010 Dec 22.
To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures.
Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and/or an increase in the time for one decimal reduction, D, and/or an increase in the time for the fourth decimal reduction, t(4D) .
Resistance of E. coli changed - increased - at all temperatures under study. Generally, the addition of TMAO to the growth medium protected E. coli cells, leading to an increase in their heat resistance, i.e. reduced k(max) and increased D and t(4D) values are obtained.
Additional knowledge on the reaction of E. coli to heat in the presence of the organic osmolyte TMAO at lethal temperatures is provided. This work contributes to an improved understanding of the level of the resistance of bacteria to heat in the presence of osmolytes.
定量分析三甲胺 N-氧化物（TMAO）对大肠杆菌 K12 MG1655 细胞在静态温度下耐热性的影响。
在 52、54 和 58°C 下使静止期大肠杆菌细胞失活。耐热性用失活速率 k(max)的降低和/或一个十位数减少所需时间 D 的增加和/或第四个十位数减少所需时间 t(4D)的增加来描述。
在所有研究温度下，大肠杆菌的抗性发生了变化——增加。通常，在生长培养基中添加 TMAO 可保护大肠杆菌细胞，从而提高其耐热性，即获得降低的 k(max)值和增加的 D 和 t(4D)值。
提供了在致死温度下存在有机渗透剂 TMAO 时大肠杆菌对热反应的更多知识。这项工作有助于更好地理解在渗透剂存在下细菌对热的抗性水平。
