Department of Molecular Bacteriology and Immunology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
FEMS Microbiol Lett. 2011 Feb;315(2):109-14. doi: 10.1111/j.1574-6968.2010.02176.x. Epub 2010 Dec 22.
The effects of detergents (cholic acid, deoxycholic acid, Triton X-100, and Nonidet P-40) on the secretion of EspB from the locus for enterocyte effacement (LEE) gene-positive Escherichia coli strains were examined. Clinical isolates of eight EPEC strains and seven STEC strains were used to detect EspB after they had been cultivated in Luria-Bertani (LB) broth containing one of the detergents. When the bacteria were cultured in LB broth supplemented with one of the detergents, the amount of EspB produced was increased by 2-32-fold depending on the detergent and the strain used. EspB was detected in all strains when they were cultured in LB broth containing all of the detergents. The results obtained in this study can be applied to immunological diagnostic methods for detecting EspB and also to the production of EspB for research purposes.
研究了清洁剂（胆酸、脱氧胆酸、Triton X-100 和 Nonidet P-40）对肠上皮细胞脱落（LEE）基因阳性大肠杆菌菌株 EspB 分泌的影响。使用了 8 株 EPEC 菌株和 7 株 STEC 菌株的临床分离株，在含有一种清洁剂的 Luria-Bertani（LB）肉汤中培养后检测 EspB。当细菌在 LB 肉汤中培养时，根据使用的清洁剂和菌株，EspB 的产量增加了 2-32 倍。当所有菌株在含有所有清洁剂的 LB 肉汤中培养时，均检测到 EspB。本研究的结果可应用于检测 EspB 的免疫学诊断方法，也可用于 EspB 的生产研究。
