与猪驼背相关的基因组区域。
Genomic regions associated with kyphosis in swine.
机构信息
USDA, ARS, U,S, Meat Animal Research Center, Clay Center, NE 68933-0166, USA.
出版信息
BMC Genet. 2010 Dec 21;11:112. doi: 10.1186/1471-2156-11-112.
BACKGROUND
A back curvature defect similar to kyphosis in humans has been observed in swine herds. The defect ranges from mild to severe curvature of the thoracic vertebrate in split carcasses and has an estimated heritability of 0.3. The objective of this study was to identify genomic regions that affect this trait.
RESULTS
Single nucleotide polymorphism (SNP) associations performed with 198 SNPs and microsatellite markers in a Duroc-Landrace-Yorkshire resource population (U.S. Meat Animal Research Center, USMARC resource population) of swine provided regions of association with this trait on 15 chromosomes. Positional candidate genes, especially those involved in human skeletal development pathways, were selected for SNP identification. SNPs in 16 candidate genes were genotyped in an F2 population (n = 371) and the USMARC resource herd (n = 1,257) with kyphosis scores. SNPs in KCNN2 on SSC2, RYR1 and PLOD1 on SSC6 and MYST4 on SSC14 were significantly associated with kyphosis in the resource population of swine (P ≤ 0.05). SNPs in CER1 and CDH7 on SSC1, PSMA5 on SSC4, HOXC6 and HOXC8 on SSC5, ADAMTS18 on SSC6 and SOX9 on SSC12 were significantly associated with the kyphosis trait in the F2 population of swine (P ≤ 0.05).
CONCLUSIONS
These data suggest that this kyphosis trait may be affected by several loci and that these may differ by population. Carcass value could be improved by effectively removing this undesirable trait from pig populations.
背景
在猪群中观察到一种类似于人类后凸的背部曲率缺陷。该缺陷范围从分体屠体中胸椎的轻度到严重弯曲，其遗传率估计为 0.3。本研究的目的是确定影响该性状的基因组区域。
结果
在杜洛克-长白-约克夏资源群体（美国肉类动物研究中心，USMARC 资源群体）中进行的 198 个 SNP 和微卫星标记的单核苷酸多态性（SNP）关联分析，为该性状提供了与 15 条染色体上的关联区域。选择了位置候选基因，特别是那些参与人类骨骼发育途径的基因，用于 SNP 鉴定。在 F2 群体（n=371）和 USMARC 资源群体（n=1257）中，16 个候选基因中的 SNP 与后凸评分进行了基因分型。SSC2 上的 KCNN2、SSC6 上的 RYR1 和 PLOD1 以及 SSC14 上的 MYST4 的 SNP 在猪的资源群体中与后凸显著相关（P≤0.05）。SSC1 上的 CER1 和 CDH7、SSC4 上的 PSMA5、SSC5 上的 HOXC6 和 HOXC8、SSC6 上的 ADAMTS18 和 SSC12 上的 SOX9 的 SNP 在猪的 F2 群体中与后凸性状显著相关（P≤0.05）。
结论
这些数据表明，这种后凸性状可能受多个基因座影响，并且这些基因座在不同群体中可能不同。通过有效去除猪群中这种不理想的性状，可以提高胴体价值。
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