Yuan Xiao-Yu, Li Yue-Hui, Qi Zhen-Hua, Peng Min-Yuan, Wan Zhi, Wang Guang-Ping, Chen Fang-Ping
Department of Hematology, Central South University, Changsha 410008, Hunan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Dec;18(6):1440-4.
The aim of this study was to investigate the effect of 3-O-acetyl-11-keto-β-boswellic acid (AKBA) on the proliferation, apoptosis and cell cycle of human acute myeloid leukemia (AML) cell line HL-60. HL-60 cells were treated by AKBA at various concentrations. The inhibitory effects of AKBA on the proliferation of HL-60 were analyzed by MTT assay. Morphologic changes of HL-60 cells were observed by fluorescence microscopy with Hochest33342 staining. Cell apoptosis rate was determined by flow cytometry with Annexin-V-FITC/PI double staining. The cell cycle was measured by flow cytometry with PI staining. The results showed that AKBA inhibited the proliferation of HL-60 and the apoptosis rate of HL-60 cells was gradually enhanced when AKBA dose increased. AKBA changed the cell cycle of HL-60, resulting in cell increase at G(1) phase and decrease at S phase. It is concluded that the AKBA has anti-proliferation and apoptosis-inducing effects on HL-60 cells, that seems a promising therapeutical approach for AML.
本研究旨在探讨3 - O - 乙酰 - 11 - 酮 - β - 乳香酸（AKBA）对人急性髓系白血病（AML）细胞系HL - 60增殖、凋亡及细胞周期的影响。采用不同浓度的AKBA处理HL - 60细胞。通过MTT法分析AKBA对HL - 60增殖的抑制作用。采用Hochest33342染色，通过荧光显微镜观察HL - 60细胞的形态变化。采用Annexin - V - FITC/PI双染法，通过流式细胞术测定细胞凋亡率。采用PI染色，通过流式细胞术检测细胞周期。结果显示，AKBA抑制HL - 60的增殖，且随着AKBA剂量增加，HL - 60细胞凋亡率逐渐升高。AKBA改变了HL - 60的细胞周期，导致G(1)期细胞增多，S期细胞减少。结论：AKBA对HL - 60细胞具有抗增殖和诱导凋亡作用，这似乎是一种有前景的AML治疗方法。
