Stem Cell and Drug Discovery Institute, Kyoto Research Park, Shimogyo-ku, Kyoto, Japan.
Cell Transplant. 2011;20(9):1423-30. doi: 10.3727/096368910X547444. Epub 2010 Dec 22.
Endothelial cells derived from human embryonic stem cells (hESC-ECs) hold much promise as a valuable tool for basic vascular research and for medical application such as cell transplantation or regenerative medicine. Here we have developed an efficient approach for the production of hESC-ECs. Using a differentiation method consisting of a stepwise combination of treatment with glycogen synthase kinase-3β (GSK-3β) inhibitor and culturing in vascular endothelial growth factor (VEGF)-supplemented medium, hESC-ECs are induced in 5 days with about 20% efficiency. These cells express vascular endothelial cadherin (VE-cadherin), VEGF receptor-2 (VEGFR-2), CD34, and platelet endothelial cell adhesion molecule-1 (PECAM-1). These hESC-ECs can then be isolated with 95% purity using a magnetic sorting system, and expanded to more than 100-fold within a month. The hESC-ECs thus produced exhibit the endothelial morphological characteristics and specific functions such as capillary tube formation and acetylated low-density lipoprotein uptake. We propose that our methodology is useful for efficient and large-scale production of hESC-ECs.
人胚胎干细胞（hESC-ECs）衍生的内皮细胞作为一种有价值的基础血管研究工具，以及细胞移植或再生医学等医学应用具有很大的应用前景。在这里，我们开发了一种高效生产 hESC-ECs 的方法。使用一种逐步组合糖原合成酶激酶-3β（GSK-3β）抑制剂处理和在血管内皮生长因子（VEGF）补充培养基中培养的分化方法，hESC-ECs 在 5 天内以约 20%的效率诱导产生。这些细胞表达血管内皮钙黏蛋白（VE-cadherin）、血管内皮生长因子受体-2（VEGFR-2）、CD34 和血小板内皮细胞黏附分子-1（PECAM-1）。然后，这些 hESC-ECs 可以使用磁性分选系统以 95%的纯度分离，并在一个月内扩增 100 多倍。如此产生的 hESC-ECs 表现出内皮细胞形态特征和特定功能，如毛细血管管形成和乙酰化低密度脂蛋白摄取。我们提出，我们的方法对于高效和大规模生产 hESC-ECs 是有用的。
