Shen Pei-hong, Zhang Wei, Zhang Yun-han, Zhang Hong-xin, Fan Qing-xia
Department of Pathology, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Bing Li Xue Za Zhi. 2010 Oct;39(10):691-4.
To investigate the inhibitory effects of siRNA targeting BCSG1 gene expression in tumor transplants of human breast cancer cell line in nude mice.
Four-pairs of small interfering RNA sequences of BCSG1 were chemically synthesized and inserted into the plasmid expression vectors, and were then transfected into human breast carcinoma cell line MCF7 by liposome method. Plasmid vector with unrelated sequence was used as the vector control. Cells transfected with 4 siRNA sequences, control vector and naive FCF7 cells were transplanted into the nude mice. The tumor inhibition was analysised. Immunohistochemical SP method and semi-quantitative RT-PCR were adopted to detect the BCSG1 mRNA and protein expression, respectively. Breast tissue samples of human infiltrating ductal carcinoma, ductal hyperplasia and fibroadenoma were also used as the controls.
The inhibition rates of tumor growth in four BCSG1-siRNA transfected groups were remarkably higher than those of the vector control group and naive MCF7 cells (P<0.01). Compared with that of the vector control and naïve MCF7 cell group, there was a significant decrease of BCSG-1 protein expression in the four experimental groups by immuno-histochemistry staining (P<0.01). In addition, BCSG1 mRNA expression in the four groups transfected with BCSG1-siRNA were significantly less than that of the control vector group, naive MCF7 cell control group and human breast IDC (P<0.01).
BCSG1-siRNA down-regulates the expression of BCSG1 and inhibits effectively growth of the transplaned human breast cancer cell line in nude mice.
研究靶向BCSG1基因表达的小干扰RNA（siRNA）对人乳腺癌细胞系裸鼠移植瘤的抑制作用。
化学合成4对靶向BCSG1的小干扰RNA序列，插入质粒表达载体，采用脂质体法转染人乳腺癌细胞系MCF7。以含无关序列的质粒载体作为载体对照。将转染4种siRNA序列、对照载体及未处理的MCF7细胞分别接种于裸鼠，观察肿瘤抑制情况。分别采用免疫组化SP法及半定量逆转录-聚合酶链反应（RT-PCR）检测BCSG1 mRNA及蛋白表达。取人浸润性导管癌、导管增生及纤维腺瘤乳腺组织标本作为对照。
4个BCSG1-siRNA转染组肿瘤生长抑制率显著高于载体对照组及未处理的MCF7细胞组（P<0.01）。免疫组化染色显示，与载体对照组及未处理的MCF7细胞组相比，4个实验组BCSG-1蛋白表达显著降低（P<0.01）。此外，4个BCSG1-siRNA转染组BCSG1 mRNA表达显著低于载体对照组、未处理的MCF7细胞对照组及人乳腺浸润性导管癌组织（P<0.01）。
BCSG1-siRNA可下调BCSG1表达，有效抑制人乳腺癌细胞系裸鼠移植瘤生长。
