Song Xue-Ru, Zhang Hui-Ying, Zhang Yan-Fang, Han Yu-Kun, Li Ke-Jun
Department of Obstetrics and Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Zhonghua Fu Chan Ke Za Zhi. 2010 Oct;45(10):767-71.
to investigate the activation of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated protein kinase (ERK) signaling pathway in the endometrium of women with polycystic ovary syndrome (PCOS) and its effect and significance in the cause of hyperplasia and carcinoma; and investigate the factors which affect the activation of the MAPK/ERK signaling pathway.
collected 52 patients diagnosed as PCOS who were taken dilation and curettage of uterus as study, while 32 non-PCOS patients matched as control group. Serum hormonal parameters, fasting blood glucose and insulin were measured in all patients. The PCOS patients were sub-group as insulin resistance group and non-insulin resistance group; all the patients were carried out pathology inspection of endometria, and the PCOS patients were sub-group as endometrial hyperplasia and carcinoma group and normal endometrium group based on the outcome of pathology inspection. Western blot were performed to detect the expressions of ERK1/2 and phosphorylated ERK1/2 (p-ERK1/2), the activation of ERK1/2.
(1) the expression of p-ERK1/2 [(61 ± 13)%] in the endometrium in PCOS group was higher than that in the control [(44 ± 10)%, P < 0.01]. (2) The expression of p-ERK1/2 was significantly increased in group of endometrial hyperplasia and carcinoma [(70 ± 11)%] compared to that in group of normal endometrium [(55 ± 10)%, P < 0.01], while there were significant difference between group of insulin resistance [(63 ± 13)%] and group of non-insulin resistance [(55 ± 7)%, P < 0.01]. (3)Fasting insulin level, insulin area under the curve and body mass index were related to the expression of p-ERK1/2 in endometrium with PCOS, the correlation coefficient were 0.447, 0.456 and 0.381, respectively (all P < 0.01).
the MAPK/ERK signaling pathway in endometrium with PCOS was overactivation, which was related to the endometrial hyperplasia and carcinoma; while the activation of MAPK/ERK signaling pathway were effected by insulin resistance and hyperinsulinemia.
探讨多囊卵巢综合征（PCOS）患者子宫内膜中丝裂原活化蛋白激酶（MAPK）/细胞外信号调节蛋白激酶（ERK）信号通路的激活情况及其在子宫内膜增生和癌变原因中的作用及意义；并研究影响MAPK/ERK信号通路激活的因素。
收集52例诊断为PCOS并行子宫刮宫术的患者作为研究对象，选取32例非PCOS患者作为对照组。检测所有患者的血清激素参数、空腹血糖和胰岛素水平。将PCOS患者分为胰岛素抵抗组和非胰岛素抵抗组；对所有患者进行子宫内膜病理检查，并根据病理检查结果将PCOS患者分为子宫内膜增生和癌变组及正常子宫内膜组。采用蛋白质免疫印迹法检测ERK1/2及磷酸化ERK1/2（p-ERK1/2）的表达，即ERK1/2的激活情况。
（1）PCOS组子宫内膜中p-ERK1/2的表达[（61±13）%]高于对照组[（44±10）%，P<0.01]。（2）子宫内膜增生和癌变组p-ERK1/2的表达[（70±11）%]明显高于正常子宫内膜组[（55±10）%，P<0.01]，胰岛素抵抗组[（63±13）%]与非胰岛素抵抗组[（55±7）%]之间也有显著差异（P<0.01）。（3）空腹胰岛素水平、胰岛素曲线下面积和体重指数与PCOS患者子宫内膜中p-ERK1/2的表达相关，相关系数分别为0.447、0.456和0.381（均P<0.01）。
PCOS患者子宫内膜中的MAPK/ERK信号通路过度激活，这与子宫内膜增生和癌变有关；而MAPK/ERK信号通路的激活受胰岛素抵抗和高胰岛素血症影响。
