Center for Molecular and Tumor Virology and Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130-3932, USA.
Virology. 2011 Feb 20;410(2):327-35. doi: 10.1016/j.virol.2010.11.020. Epub 2010 Dec 21.
The 150 kbp genome of equine herpesvirus-1 (EHV-1) is composed of a unique long (UL) region and a unique short (Us) segment, which is flanked by identical internal and terminal repeat (IR and TR) sequences of 12.7 kbp. We constructed an EHV-1 lacking the entire IR (vL11ΔIR) and showed that the IR is dispensable for EHV-1 replication but that the vL11ΔIR exhibits a smaller plaque size and delayed growth kinetics. Western blot analyses of cells infected with vL11ΔIR showed that the synthesis of viral proteins encoded by the immediate-early, early, and late genes was reduced at immediate-early and early times, but by late stages of replication reached wild type levels. Intranasal infection of CBA mice revealed that the vL11ΔIR was significantly attenuated as mice infected with the vL11ΔIR showed a reduced lung viral titer and greater ability to survive infection compared to mice infected with parental or revertant virus.
马疱疹病毒 1 型（EHV-1）的 150 kbp 基因组由一个独特的长（UL）区和一个独特的短（Us）片段组成，其两侧为 12.7 kbp 的相同内部和末端重复（IR 和 TR）序列。我们构建了一个缺失整个 IR 的 EHV-1（vL11ΔIR），并表明 IR 对于 EHV-1 的复制是可有可无的，但 vL11ΔIR 的噬菌斑尺寸较小，生长动力学延迟。用 vL11ΔIR 感染的细胞的 Western blot 分析表明，立即早期、早期和晚期基因编码的病毒蛋白的合成在立即早期和早期减少，但在复制的晚期达到野生型水平。CBA 小鼠的鼻腔感染表明，vL11ΔIR 显著减毒，因为感染 vL11ΔIR 的小鼠的肺部病毒滴度降低，并且与感染亲本或回复病毒的小鼠相比，具有更强的感染生存能力。
