Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
J Hazard Mater. 2011 Feb 28;186(2-3):1271-6. doi: 10.1016/j.jhazmat.2010.11.126. Epub 2010 Dec 4.
Microbe-assisted phytoremediation is emerging as one of the most effective means by which plants and their associated rhizosphere microbes degrade organic contaminants in soils. A pot study was conducted to examine the effects of inoculation with Rhizobium meliloti on phytoremediation by alfalfa grown for 90 days in an agricultural soil contaminated with weathered polycyclic aromatic hydrocarbons (PAHs). Planting with uninoculated alfalfa (P) and alfalfa inoculated with R. meliloti (PR) significantly lowered the initial soil PAH concentrations by 37.2 and 51.4% respectively compared with unplanted control soil. Inoculation with R. meliloti significantly increased the counts of culturable PAH-degrading bacteria, soil microbial activity and the carbon utilization ability of the soil microbial community. The results suggest that the symbiotic association between alfalfa and Rhizobium can stimulate the rhizosphere microflora to degrade PAHs and its application may be a promising bioremediation strategy for aged PAH-contaminated soils.
微生物辅助植物修复作为一种最有效的方法之一，正在兴起，通过植物及其相关的根际微生物来降解土壤中的有机污染物。进行了一项盆栽研究，以研究接种根瘤菌（Rhizobium meliloti）对在风化多环芳烃（PAHs）污染的农业土壤中生长 90 天的紫花苜蓿进行植物修复的影响。与未种植对照土壤相比，未接种苜蓿（P）和接种根瘤菌（PR）的苜蓿种植分别显著降低了初始土壤 PAH 浓度 37.2%和 51.4%。接种根瘤菌显著增加了可培养的 PAH 降解细菌的数量、土壤微生物活性和土壤微生物群落的碳利用能力。结果表明，苜蓿与根瘤菌之间的共生关系可以刺激根际微生物区系降解 PAHs，其应用可能是一种很有前途的生物修复策略，适用于老化的 PAH 污染土壤。
