College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, Shandong Province 266042, PR China.
Bioresour Technol. 2011 Feb;102(4):3805-9. doi: 10.1016/j.biortech.2010.11.092. Epub 2010 Nov 26.
This study determined hydrogen production, volatile fatty acids (VFAs) generation and cellulose solubilisation from anaerobic dark fermentation of wheat stalk and showed the effect of different mixed microflora. The cumulative hydrogen yields of anaerobic digested activated sludge (AS)-inoculated and anaerobic digested dairy manure (DM)-inoculated system were 23.3 and 37.0 mL/g VS at 204 h, respectively. A modified Gompertz equation was able to adequately describe the production of hydrogen from the batch fermentation by both mixed microflora. During the process, acetate and butyrate accounted for more than 76.1% of total VFAs for both fermentations. The extent of cellulose solubilisation approached 46.6% and 75.2% for AS- and DM-inoculated fermentation, respectively. The X-ray diffraction (XRD) showed that the crystallinities of both fermented stalks were partly disrupted by the mixed microflora, and DM-inoculated fermentation had more disruption than AS-inoculated one.
本研究通过厌氧暗发酵小麦秸秆，确定了产氢量、挥发性脂肪酸（VFAs）生成和纤维素溶解情况，并展示了不同混合微生物群落的影响。接种厌氧消化活性污泥（AS）和接种厌氧消化奶牛粪便（DM）的系统在 204 小时时的累积氢气产量分别为 23.3 和 37.0 mL/gVS。修正的 Gompertz 方程能够充分描述两种混合微生物群落的批式发酵产氢过程。在此过程中，两种发酵的总 VFAs 中乙酸和丁酸均占 76.1%以上。AS 和 DM 接种发酵的纤维素溶解程度分别接近 46.6%和 75.2%。X 射线衍射（XRD）表明，混合微生物群落部分破坏了发酵秸秆的结晶度，DM 接种发酵的破坏程度大于 AS 接种发酵。
