Nakayama H, Takahashi T, Nakagawa-Mizuyachi K, Kawashima M
The United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Japan.
Poult Sci. 2011 Jan;90(1):191-4. doi: 10.3382/ps.2010-00965.
The present study was performed to elucidate whether estradiol-17β (E₂) would affect calcitonin (CT) receptor binding in the hen neurohypophysis. The equilibrium dissociation constant (K(d)) and the maximum binding capacity (B(max)) of the CT receptor in the plasma membrane fraction of the hen neurohypophysis were examined by Scatchard analysis of specific binding of (125)I-labeled chicken CT. A single i.m. injection of E₂ into nonlaying hens caused a decrease in K(d) and B(max) values of the CT receptor. The K(d) and B(max) values of the CT receptor were smaller in laying hens than in nonlaying hens. The present study suggests that E₂ may increase the action of CT on the neurohypophysis in hens.
本研究旨在阐明17β-雌二醇（E₂）是否会影响母鸡神经垂体中降钙素（CT）受体的结合。通过对¹²⁵I标记的鸡降钙素特异性结合进行Scatchard分析，检测了母鸡神经垂体质膜部分中CT受体的平衡解离常数（K(d)）和最大结合容量（B(max)）。对不产蛋母鸡单次肌肉注射E₂导致CT受体的K(d)和B(max)值降低。产蛋母鸡中CT受体的K(d)和B(max)值比不产蛋母鸡小。本研究表明，E₂可能会增强CT对母鸡神经垂体的作用。
