Noreĭko B V, Gurenko E G
Probl Tuberk. 1990(5):48-50.
Bacteriological examination of sputum smears of 42 patients with anthracotic tuberculosis divided according to the activity of a tuberculous process into 2 groups was undertaken. To achieve the growth of L-forms, sputum inoculation was done on Shkolnikova's semiliquid culture medium. Phase contrast microscopic examination of the sputum smears was carried out at 280 X magnification. As the pneumoconiotic process in patients with anthracotic pulmonary tuberculosis grows progressively worse, the rate of typical Mycobacterium strain positivity decreases, while the rate and intensity of L-forms excretion proportionally increase. In the patients with inactive forms of anthracotic tuberculosis, the bacillary excretion was characterized by monomorphism of vacuolized L-forms. At the same time, active forms of anthracotic tuberculosis were accompanied by a massive excretion of Myco. L-forms and featured a wide range of morphologic changes dominated by giant cells, cord-factor, heterogeneous granulation and homogeneous mass as a consequence of overall destruction of the cell membranes.
对42例煤工尘肺结核患者的痰液涂片进行了细菌学检查,根据结核病变活动程度将患者分为2组。为使L型菌生长,将痰液接种于什科尔尼科娃半液体培养基上。痰液涂片在280倍放大倍数下进行相差显微镜检查。随着煤工尘肺结核患者的尘肺病变逐渐加重,典型分枝杆菌菌株阳性率降低,而L型菌排出率和强度则成比例增加。在非活动性煤工尘肺结核患者中,细菌排出以空泡化L型菌的单形性为特征。同时,活动性煤工尘肺结核伴有大量的分枝杆菌L型菌排出,其形态学变化范围广泛,以巨细胞、索状因子、异质性颗粒和均质团块为主,这是细胞膜全面破坏的结果。