Enomoto N, Takada A, Nakao T, Date T
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
Biochem Biophys Res Commun. 1990 Aug 16;170(3):1021-5. doi: 10.1016/0006-291x(90)90494-8.
The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype.
采用聚合酶链反应（PCR）检测慢性非甲非乙型肝炎患者血浆中的丙型肝炎病毒（HCV）。通过选择合适的引物，24份样本中有19份（79%）呈阳性。对编码NS5基因一部分的400 bp cDNA扩增片段进行序列分析表明，在日本HCV可分为两种类型（命名为K1和K2）。对这些片段进行狭缝印迹杂交表明，13份为HCV-K1，6份为HCV-K2，它们与原型的核苷酸序列同源性分别为80%和67%。
